FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3848064
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1652344.293951451950903TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.1475547184246416No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA41380.1075345940192263TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG193850.064.68716428
GTATGCC194300.064.1067247
ACTCCAG199000.063.0724123
CGTATGC198250.062.86474246
GTCACGA199550.062.82188429
TCGTATG198000.062.78503845
TGAACTC199850.062.73409720
CATCTCG193850.062.6518141
CAGTCAC200350.062.64090327
GCACACG201600.062.58234411
GAACTCC201700.062.2107621
CGAGATT200950.062.14845733
CACGAGA201550.062.1189431
CCAGTCA201850.062.0935526
CACACGT203200.062.0206812
CTCCAGT202500.061.9304224
TCTGAAC203750.061.684718
TCACGAG203350.061.6543430
ACGTCTG204450.061.5232615
TCCAGTC204100.061.51192525
Produced by FastQC (version 0.11.5)