FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220293
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1340546.037671604603537TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG153600.065.27946528
CATCTCG148950.064.753241
GTATGCC154800.064.48096547
CAGTCAC155950.064.2957727
CACACGT157900.064.0129812
GAACTCC156950.063.9350321
GCACACG158350.063.83106611
TCCAGTC156950.063.8235325
ACACGTC158200.063.80309713
CTCCAGT157150.063.7645824
CCAGTCA157100.063.7403126
TCGTATG156350.063.7283645
CGAGATT157400.063.66329633
CACGTCT158700.063.5800214
GCCGTCT154700.063.57245351
GTCACGA157950.063.5259629
ACTCCAG157950.063.44161623
CGTATGC157500.063.32970446
TGAACTC158700.063.29617720
CCATCTC152550.063.24804340
Produced by FastQC (version 0.11.5)