FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027105
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1183842.939679993444422TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG134550.064.5241341
GTCACCT138200.064.14657629
GTATGCC137400.063.91111847
ACTCCAG138950.063.7791123
CTATCTC137250.063.1527840
CACACGT141950.063.0691312
AGTCACC140850.063.01502628
CCAGTCA140900.062.9701726
GAAGCTA140500.062.66444836
TGAACTC142050.062.60821520
GCACACG143050.062.6078411
CGTATGC140500.062.55002246
CAGTCAC142600.062.36441427
TCGTATG141000.062.35304345
AGCTATC141050.061.57625238
CTCCAGT144750.061.3202524
GAACTCC144950.061.2348921
AGAGCAC146650.061.2141238
AGCACAC147950.060.6289410
AACTCCA146400.060.60523622
Produced by FastQC (version 0.11.5)