FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4928912
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3189456.470900677472026TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT119050.24153403428586268TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA62880.12757379316165515TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT54530.11063293481401168TruSeq Adapter, Index 7 (97% over 35bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC50860.10318707252229295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG360050.065.4889641
AGTCACG411350.064.71342528
CGTATCT378150.064.4655839
GTATGCC364750.064.42416447
GAACTCC414100.064.3688721
GTATCTC369350.064.2386940
ATTCGTA410750.064.2317636
GCACACG418550.064.092911
GTCACGA415850.064.0209129
ACTCCAG416750.063.9356723
ACACGTC419600.063.9241813
TCGTATG353950.063.9178545
CTCCAGT416750.063.86847324
CACACGT420450.063.81159612
TGAACTC418850.063.74753620
TCCAGTC418350.063.6634625
AACTCCA419000.063.65917222
CAGTCAC418550.063.64201427
CGTATGC362950.063.6057846
CCAGTCA418550.063.59958326
Produced by FastQC (version 0.11.5)