FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT83960.20991931257675706TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG15150.038.81684541
GCACACG15900.037.42008611
CACACGT16200.037.15920612
ACACGTC16650.035.9451513
CACGTCT18300.032.89544714
ACCGCTC18300.031.74912332
AGTCACC18200.031.72966828
CAGTCAC18750.031.35891327
CCAGTCA19250.030.3637226
AGCACAC20350.029.58128410
CTCCAGT20300.029.31079524
TCCAGTC20350.029.23841325
ACTCCAG21200.028.56175823
GTATGCC20850.028.3690347
TCGTATG20850.028.3690345
AGAGCAC21250.028.3280758
GAACTCC20950.028.23467621
CGTATGC21250.027.6703246
TGAACTC21950.026.94835920
CACCGCT22150.026.54668431
Produced by FastQC (version 0.11.5)