FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190830
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT574621.800848055208206TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC69300.064.74731447
TATCTCG69400.064.10931441
ACTCCAG71500.063.29454423
CACACGT71850.062.88583812
GCACACG72550.062.423811
TGAACTC72800.062.26239420
TCGTATG72800.061.77869445
AGTCACC73000.061.7033928
CGTATGC73400.061.0829646
ACCGCTC73350.061.0282132
CAGTCAC74000.060.96415727
CCAGTCA74300.060.7670126
CACCGCT74250.060.52415531
AGAGCAC76200.059.4336858
ATTATCT75150.059.1565739
AACTCCA77000.058.86533722
GAACTCC77000.058.82080521
TCTGAAC77550.058.7186518
CTCCAGT77350.058.59805724
CTCATTA76700.058.32620636
Produced by FastQC (version 0.11.5)