FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021358
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT36449812.064045372974668TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA53730.17783394089677557TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG42290.1399701723529618TruSeq Adapter, Index 7 (97% over 35bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA36920.12219670757321707No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC33060.10942099545965754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT418250.065.7866212
GTATGCC413900.065.7593147
GCACACG418550.065.7478411
CCGGCTA412700.065.68349533
CACGTCT418650.065.6819714
ACGTCTG418750.065.6757315
CTCCAGT415950.065.6604924
AGTCACC414550.065.5886628
ACACGTC419750.065.58488513
TGAACTC418300.065.5844820
TCCAGTC416000.065.5768825
GAACTCC417800.065.5456821
TCGTATG414600.065.53853645
GCCGTCT413900.065.4802751
GTCTGAA420000.065.463617
CCAGTCA416750.065.4504626
GGCTATG414750.065.38978635
TCTGAAC420300.065.3585818
AACTCCA419050.065.3501622
CGTATGC417000.065.3208146
Produced by FastQC (version 0.11.5)