FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864892
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT706281.8274249319256526TruSeq Adapter, Index 13 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39250.10155523103879745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC98050.063.36662321
GCACACG101550.062.03553811
ACGTCTG101800.061.50581415
TGAACTC101600.061.35924520
ACTCCAG101550.061.3197223
GTCACAT101600.061.31606329
GTATGCC86650.060.95007747
CACGTCT103600.060.63987414
AGCACAC104100.060.58317610
ACACGTC104200.060.39146413
AGTCACA103550.060.36418528
AACTCCA104200.059.861822
CACACGT105550.059.6190512
CTCCAGT104400.059.6122424
CACATTC104150.059.5834331
CTCGTAT86800.059.55447444
TCGTATG86050.059.54479245
TCCAGTC104600.059.46402425
CGTATGC89200.058.38371346
TCTGAAC107200.058.34378418
Produced by FastQC (version 0.11.5)