FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723773
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT38230.22178094215421634TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC7450.041.34550521
CTCCAGT7550.040.79787424
TGAACTC8000.038.50300220
ACACGTC8000.038.4985313
CACGTCT8050.038.2594114
CACACGT8250.037.75613812
AGTCACA8700.035.40094828
GTCACAT8800.034.99765429
GCACACG8950.034.80213511
ACTCCAG8900.034.60943623
CACATTC9250.033.67439331
CGTATGC8400.032.91732446
TCCAGTC9750.032.30927325
GTATGCC8850.031.63996547
ACGTCTG9950.031.30624615
AATCTCG8200.031.16096341
GCCGTCT7550.030.13217451
CCAGTCA10350.030.0980926
AGCACAC10350.030.09459710
AACTCCA10450.029.81093422
Produced by FastQC (version 0.11.5)