FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649405
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2152095.8970982941054775TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT78360.21471993379742726TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG271800.066.8292128
GAACTCC274100.066.5292821
CTCCAGT274400.066.3289824
ATTCGTA272450.066.1208636
GTATGCC243500.066.0451447
TCCAGTC275400.066.0236925
TCGTATG237000.065.862945
CTCGTAT241500.065.85344
TATCTCG248000.065.8367441
CCAGTCA277150.065.6951926
ACTCCAG277300.065.6605623
CACACGT279650.065.6290712
GTCACGA277150.065.56441529
CGTATCT256950.065.5590339
ACGTCTG279200.065.5360815
CGTATGC241050.065.4822846
CACGTCT280000.065.4220414
GTATCTC251100.065.41341440
TGAACTC279150.065.3507920
TTCGTAT274750.065.32526437
Produced by FastQC (version 0.11.5)