FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712958
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT619081.0836417841685515TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG434030.759729023038503TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT121690.2130069921746318TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.1044467682065928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT65150.062.74616644
GAACTCC154750.062.6304121
GTCACTC154550.062.52316329
TCGGGGG115700.062.2534143
AGTCACT157950.061.31078328
GCACACG158350.061.2879911
TCCAGTC158600.061.0207225
ACGTCTG159600.060.80850615
ACACGTC160200.060.64629713
CTCCAGT159650.060.6423824
ACTCCGG159550.060.43695432
TGAACTC160500.060.4302620
CTCCGGA160100.060.18560833
ATCGGGG144550.059.82818242
CACTCCG162550.059.40766531
CACGTCT163750.059.3101514
AACTCCA163750.059.14538222
CAGTCAC163950.059.13106527
TCCGGAG162800.059.10351634
ACTCCAG165000.058.69730823
Produced by FastQC (version 0.11.5)