FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175335
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT38310.17611080592184652TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG29380.1350596574780436TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT5650.049.5574444
TCGGGGG9650.049.32520743
ACTCCGG13200.047.99234432
GTCACTC13750.046.3261329
ATCGGGG12650.045.3743842
CTCCGGA14500.043.68958733
CACTCCG14750.043.66094631
CTCCAGT14900.042.98848324
GAACTCC15300.041.864621
CACGTCT15450.041.4552914
TCCGGAG15250.041.31235534
CGGGGGG4900.039.99993544
ACGTCTG16150.039.2250415
ACACGTC16300.039.07879313
AGTCACT16750.038.6557628
CCGGAGA16400.038.41721735
CCAGTCA16800.038.33415226
GCACACG16650.038.25819411
CAGTCAC17550.037.6913827
TGAACTC17450.036.9070820
Produced by FastQC (version 0.11.5)