FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_OAT0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_OAT0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562640
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT810075.183983515077049TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG565173.6167639379511596TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT168921.08099114319357TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT44910.2873982491168791TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTG31790.20343777197563095TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTGT23280.14897865151282444TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG178250.066.817742
CACTCCG199200.066.6778531
GTGGGTG40250.066.6073259
TCGGGGG148500.066.6046843
TCACTCC199700.066.59852630
CTCCAGT200600.066.59846524
CGGGGGT86150.066.5881144
CTCCGGA199300.066.5565833
TGAACTC201300.066.5407520
GAACTCC201150.066.5207721
AGTCACT201000.066.4812228
ACTCCAG201150.066.4337723
GTCACTC200900.066.427229
TCCAGTC201500.066.33362625
ACGTCTG202400.066.3174615
CCAGTCA201750.066.2340826
AATCGGG186550.066.0150741
AACTCCA202850.065.9805422
CAGTCAC202800.065.9601827
CACACGT204050.065.847712
Produced by FastQC (version 0.11.5)