FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T4.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T4.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854251
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT562711.4599723785503331TruSeq Adapter, Index 13 (97% over 38bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG42640.1106310927856022No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG41340.10725819361530943No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA40870.10603876083835743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG83100.059.51326423
CACACGT84650.058.9166412
AGTCACA84850.058.3240528
GAACTCC85700.057.87109421
CCAGTCA85750.057.75571426
AACTCCA85700.057.7485722
TGAACTC86350.057.5570620
ACACGTC87300.057.20840513
GTATGCC73150.057.0333547
TCCAGTC87050.057.01381325
GCACACG88350.056.6480811
CTCCAGT87950.056.3905924
CAGTCAC88350.056.0135327
AATCTCG75150.055.99067741
GTCACAT88300.055.88670329
CACGTCT89650.055.82591214
ACGTCTG90050.055.50092315
CACATTC89150.055.47450631
TCGTATG71900.055.29817645
CTGAACT90150.055.20713419
Produced by FastQC (version 0.11.5)