FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794795
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1531323.193713182732526TruSeq Adapter, Index 27 (97% over 39bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG184490.384771403156965No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA126890.26464113689949204No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC118670.24749754681899855No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG114620.23905088747276995No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC92620.19316779966609626No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA85230.1777552533528545No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC73600.15349978466232653No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC64570.1346668627125873No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT62060.1294320195128259No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG58510.122028157616749No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG53740.11207986994230203No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT51920.10828408722374994No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG48010.10012941116356383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA173750.064.18510436
ACTCGAT173900.063.68691337
ACATTAC176400.063.6104432
GTATGCC176500.063.55523347
ATTACTC178200.062.81405634
CATTACT178750.062.7741633
CTCGTAT176800.062.7347244
AGTCACA180050.062.6299828
TCGATCT175700.062.3371739
CGTATGC180150.062.18982746
TTACTCG180000.062.0342435
TCGTATG180850.061.98782745
ACTCCAG181800.061.89880423
CACATTA182200.061.7199831
GTCACAT182750.061.62806729
CCAGTCA184500.060.98978426
CAGTCAC185050.060.80534427
TATGCCG184650.060.7683848
GCCGTCT183300.060.73668751
GAACTCC185400.060.71576721
Produced by FastQC (version 0.11.5)