FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717377
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT277210.7457139805836211TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC37300.054.2450940
TGAACTC38950.053.2888220
GTATGCC38450.053.24994747
AGTCACC39150.052.8335228
GTCACCT39200.052.76612529
TATCTCG38500.052.73760241
GAACTCC39650.052.4363121
GAAGCTA40550.051.45273636
ACTCCAG41450.050.41186523
TCGTATG41000.050.1087845
CACACGT41750.050.04625712
GCACACG42050.049.85511
CTCCAGT42400.049.19980224
CCAGTCA42400.049.03470226
TCCAGTC43050.048.61955625
AGCTATC42100.048.47742538
AAGCTAT42600.048.40149737
CGTATGC42900.047.97109646
GCTATCT43100.047.1895839
CAGTCAC44950.046.7164127
Produced by FastQC (version 0.11.5)