FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415905
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT458901.0391980805746501TruSeq Adapter, Index 19 (97% over 38bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG103440.23424416965491784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC57600.059.06876840
TATCTCG58500.058.2218341
GTATGCC59450.057.99080747
AGTCACC60050.057.8161128
GTCACCT60450.057.4914429
ACTCCAG60700.057.0849123
AGCTATC61150.055.86856538
CCAGTCA62400.055.75343726
CAGTCAC62850.055.40680327
GAAGCTA63900.054.1763336
TCGTATG63950.054.07433345
AAGCTAT63900.053.84765637
GAACTCC64600.053.80114721
CACACGT65100.053.70868712
TGAACTC65600.053.1410720
GCACACG66200.052.97425511
CTCCAGT65850.052.83301524
CGTATGC66800.051.71487446
TCCAGTC67900.051.18578325
GCTATCT67300.050.71117839
Produced by FastQC (version 0.11.5)