FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126017
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2189595.306788605088151TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC254200.066.0206147
CTATGAT250700.065.85058637
CCGGCTA254700.065.58948533
AGTCACC255800.065.45318628
ACTCCAG257450.065.3814723
CCAGTCA256950.065.3019926
TCGTATG257600.065.1212645
CGTATGC258300.065.0531846
CTCCAGT258700.064.9843724
CAGTCAC259300.064.7181727
GGCTATG257900.064.71835
GCTATGA258350.064.4155736
CTCGTAT257150.064.3233144
TCACCGG259550.064.3226330
GAACTCC261550.064.26366421
TCCAGTC261950.064.14905525
TGAACTC262300.064.0799120
CACACGT263300.064.0680612
GCACACG265250.063.5970611
CCGTCTT262700.063.4683352
Produced by FastQC (version 0.11.5)