FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458579
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2106274.724083614981365TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC246500.064.3762647
ACTCCAG247450.064.1178423
CACACGT250400.063.9018712
CCAGTCA248200.063.8669726
GAACTCC248850.063.8274521
GCACACG250750.063.82591611
CAGTCAC249200.063.76017427
CTATGAT246250.063.7474137
AGTCACC248650.063.68936528
TCGTATG248950.063.68647845
GCTATGA249150.063.25834336
TGAACTC251550.063.23975820
CTCCAGT251100.063.1021924
ACGTCTG253400.063.07698415
CCGGCTA251400.062.9461833
GGCTATG251200.062.93720635
CGTATGC252750.062.78436746
AGCACAC256100.062.54723410
ACACGTC256050.062.5054813
AACTCCA255050.062.37193322
Produced by FastQC (version 0.11.5)