FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956712
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1243203.1420027538016413TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT53630.13554183372456727TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA167300.064.68391428
CCAGTCA168700.063.87983726
ACTCCAG169350.063.7586623
GTATGCC142500.063.71003347
CGTATGC140700.063.35597646
CAGTCAC171250.063.0284427
TCGTATG139750.062.9602245
CACACGT173650.062.82243712
GCACACG173800.062.68845711
GAACTCC173000.062.5146221
TGAACTC174250.062.2469420
GTCACAT173850.062.1864829
CTCCAGT174000.061.9541924
TCCAGTC174600.061.76133325
AACTCCA175250.061.73198322
CACATTC175300.061.67631
AATCTCG148850.061.6378941
TCTGAAC178100.061.19457618
ACGTCTG178650.061.0845415
CGTCTGA179650.060.80296716
Produced by FastQC (version 0.11.5)