FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T0_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T0_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4103057
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT287940.7017694367882289TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC37350.057.5481540
TATCTCG38750.055.64966641
GAACTCC41700.052.62856721
AGTCACC41850.052.43545528
GCTATCT41050.052.277139
GTCACCT42350.051.81575829
GTATGCC41950.051.55833447
CACACGT44300.050.0083412
ACACGTC44450.049.7608413
CACGTCT45000.048.9971114
CCAGTCA45100.048.7386126
GAAGCTA44850.048.5503236
CTCGTAT45200.048.3931644
CTCCAGT45700.048.25189624
AGCTATC45000.047.92171538
ACTCCAG46250.047.90571223
GCACACG46350.047.8720411
TGAACTC46250.047.52615420
TCGTATG46250.047.1431645
CGTATGC46700.046.98866346
Produced by FastQC (version 0.11.5)