FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477288
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT519951.4952744782715726TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT138970.3996505322538714TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGTT95420.27440925226785934TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGAT91130.2620720515528193TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGAT81590.23463687793475835TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTGGTAT75920.2183310671994957TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTAT66330.19075210336331072TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGAT55390.15929080363777748TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGGT50500.14522812030524937TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTTT43690.12564389259675932TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGTTT41090.11816680125431084TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG184000.064.330633
GAACTCC187350.063.89019421
AGTCACT187500.063.66925428
GTCACTC187600.063.48606529
GCACACG188900.063.3844811
ACTCCGC187750.063.0829832
AACTCCA190050.062.96410422
CAGTCAC190050.062.90704327
ACACGTC190800.062.8816913
ACTCCAG190300.062.7876223
CACACGT191200.062.75014512
ACGTCTG191350.062.62778515
CTCCAGT190900.062.5536124
TCCAGTC191050.062.4129125
CACTCCG190400.062.31438431
CGCGAAA189600.062.2787336
CACGTCT192600.062.2213314
TCACTCC191100.062.049530
CCAGTCA192700.061.8784926
TCTGAAC194450.061.7373518
Produced by FastQC (version 0.11.5)