FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860492
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT115310.4031124715608364TruSeq Adapter, Index 1 (97% over 36bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG82930.289915161447751No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC35360.12361509838167699No Hit
GTGAACTACTGTGGAAGTTACTGACGTAAGATTACGCCGCGTGAATACCG34250.11973464704673183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG16400.052.7207535
TAGATCT16100.052.61601339
AGTCACA17100.051.37243328
CGATAGA16750.051.20115336
ACACGTC18500.048.05233813
GCACACG18550.047.54547511
CAGTCAC18600.047.4176627
CACACGT18700.047.16409312
GAACTCC18850.046.79205321
ATAGATC18300.046.67315738
CCAGTCA18900.046.48307426
AGATCTC18500.045.97856540
CTCCAGT19450.045.1686424
CACGTCT19800.045.0741514
CGTATGC19750.044.12500446
TCCAGTC20000.043.92650625
GTCACAG20000.043.92343529
CACAGCG20150.043.7724431
GCCGTCT20100.043.5307851
ACTCCAG20300.043.2773423
Produced by FastQC (version 0.11.5)