FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666807
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT4443799.522120799081685TruSeq Adapter, Index 27 (97% over 39bp)
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG60400.12942467944356817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA482750.067.0919836
ACTCGAT478850.067.0396637
ATTACTC486900.066.7372934
ACATTAC488800.066.6941632
GTATGCC487150.066.6434647
TTACTCG487850.066.5771635
AGTCACA491850.066.4250428
CCAGTCA491050.066.378626
ACTCCAG492350.066.246723
CTCCAGT492750.066.136124
TCGTATG490400.066.1304345
GAACTCC494050.066.1179321
CGTATGC491200.066.072646
TCGATCT479350.066.0349339
CATTACT493350.066.00812533
CAGTCAC494750.065.98617627
CACACGT498650.065.9052412
CACATTA495450.065.88376631
GCACACG499300.065.88183611
TCTCGTA483500.065.80881543
Produced by FastQC (version 0.11.5)