FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_79T-1_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_79T-1_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096000
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT2765346.7513183593749995TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT297850.066.3566837
TACTCGA300600.066.3427336
GTATGCC304800.065.796347
TTACTCG305150.065.51411435
ATTACTC306250.065.3867134
ACATTAC308150.065.23025532
AGTCACA309650.065.1710628
GAACTCC309950.065.11513521
CCAGTCA309800.065.0788726
TCGTATG308650.064.92943645
TCGATCT301550.064.892439
TCTCGTA303250.064.87379543
CATTACT310000.064.7280833
TGAACTC312350.064.6932520
CTCGTAT306500.064.68754644
GTCACAT311750.064.66469629
CAGTCAC312000.064.63531527
CGTATGC310950.064.5504746
CTCCAGT312900.064.46767424
TCCAGTC313350.064.3527525
Produced by FastQC (version 0.11.5)