FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_659T0_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_659T0_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734434
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT208651.2029861038240717TruSeq Adapter, Index 13 (97% over 38bp)
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA24980.14402392942020278No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA22850.1317432661029477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC32050.059.73862521
AATCTCG28550.058.48517241
CTCCAGT33200.057.87855524
AGTCACA33300.057.59465828
GCACACG33600.057.28874611
GTATGCC28550.057.2491647
ACACGTC33600.057.1862313
TCCAGTC33700.057.12203625
CACACGT33750.056.93206812
CACGTCT34000.056.7193314
ACTCCAG34050.056.43371223
CCAGTCA34250.056.1025626
CTCGTAT29400.055.71304744
CGTATGC29550.055.54867646
AGCACAC34750.055.49357210
GTCACAT34550.055.30831529
TGAACTC35000.055.10354220
CAGTCAC35000.054.99719227
AACTCCA35350.054.26091422
TCGTATG30000.054.1321345
Produced by FastQC (version 0.11.5)