FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_659T-1_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_659T-1_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529469
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT326340.72048180482083TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG231460.5110091271184326TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT62310.13756579413613385TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG64500.064.4094443
ATCGGGG79750.061.3967742
ACTCCGG89900.059.95683732
CGGGGGT40100.059.08847844
GTCACTC91700.058.81485729
CACTCCG91900.058.72818431
TCACTCC92050.058.59446330
GAACTCC92950.058.1764521
AGTCACT93500.057.79488828
TCCGGAG94100.057.43144634
CTCCGGA94100.057.35515233
CTCCAGT94500.057.29630324
CCAGTCA95400.056.90252326
ACTCCAG95900.056.60584623
CACACGT96300.056.3694812
CAGTCAC96050.056.33338527
TGAACTC96550.056.11601320
ACACGTC97000.055.96269213
CACGTCT97100.055.68878614
GCACACG97950.055.34845411
Produced by FastQC (version 0.11.5)