FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7112719
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133128318.716935112999682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331250.4657150099701675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100080.14070568512547732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT28900.028.6898066
AAGATAT12100.025.1590254
CGATCTT42750.024.880415
TAAGATA9800.022.1343773
TTCCGGT5850.022.127182
GTCTTAC3600.021.3778271
GATATAT4350.020.9103456
TCCAATC8550.020.4599973
TCCGGTC6000.020.4088463
CTTGTCT2950.020.1734499
TAGTTCT1751.2711462E-719.9950244
CTTCCGG8250.019.505061
GCATGTT2705.456968E-1219.4591220
TCTAGCG1801.7017919E-719.4489448
ATCTAGC4950.019.0914157
TATACGG1107.4995036E-419.0844045
TACGATA4050.019.0050623
TAGGGCA2851.2732926E-1118.42414528
ATCTTTT48300.018.2613547
TCGTGTA3850.018.19039515
Produced by FastQC (version 0.11.5)