FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7112719
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94690413.312827344929556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418000.5876796201283926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT196350.028.311166
CGATCTT216850.027.827415
ATCTTTT222400.024.6615477
CTTTTTT235400.023.470949
CCTAGGG3300.022.2554992
TTCCGAT461300.021.2657852
CCGATCT461700.021.142494
ATCTGGG12150.020.9894987
TCCGATC468050.020.9382613
TTCGGAA6100.020.6397612
CTTCCGA486300.020.3465861
CTAGGGT1901.3747922E-820.241364
TCTTTTT275450.020.1652558
TAGGACT6250.019.0195164
GTGCTCT339300.018.591191
TCTTCCG345900.018.2742395
CTAGCGT1150.001019109218.2330159
GTGTATA1552.0058058E-518.0447036
GATCTGG33200.018.0073346
AGGACTA6250.017.9003455
Produced by FastQC (version 0.11.5)