H3W35AFXY_n01_adR3wtZT14s59.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adR3wtZT14s59.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172595
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	388	0.22480373127842637	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	364	0.21089834583852368	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	316	0.18308757495871839	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	267	0.1546974130189171	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	266	0.15411802195892116	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	246	0.14253020075900227	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	238	0.13789507227903475	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAAGT	15	0.0022216926	70.0	1
AAGTACA	20	0.006942079	52.5	33
TAATAGG	20	0.006942079	52.5	6
TCCTATT	20	0.006942079	52.5	2
AATAGAC	20	0.006942079	52.5	29
GCGTCTA	20	0.006942079	52.5	13
GCCGTCT	45	9.1694266E-5	38.888885	46
CCTAGCT	40	0.002413759	35.0	32
CAACATA	40	0.002413759	35.0	48
TCCTAGC	40	0.002413759	35.0	31
ATCTTTT	285	0.0	33.157894	7
TCTTTTT	300	0.0	32.666668	8
TATGGCG	55	2.9829974E-4	31.81818	5
GTATGCC	55	2.9829974E-4	31.81818	42
AACAGTC	55	2.9829974E-4	31.81818	47
TTAATAG	45	0.0042984188	31.111109	5
CTTTTTT	315	0.0	29.999998	9
GTACTGG	60	4.9669656E-4	29.166666	1
CTTAATA	50	0.0071936646	27.999998	4
GATCGTT	50	0.0071936646	27.999998	18