FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8574220
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6122277.140322968153372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131460.15332006876427245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT59900.028.0538376
TAGTCTC1154.9438313E-827.37315
CGATCTT80250.025.1483825
GCCGTAT6750.024.38087846
CGTATCA6900.023.85349348
CCGTATC7100.023.18092347
CAGAGTA2500.022.35754618
ATCTTTT78700.021.6945787
GTCCTAG4950.021.4074341
GTATCAT7600.021.19670749
GATCTGG30250.020.7884046
TTCCGGT4800.019.7934512
TAGGACT7050.019.3668464
TACGATA5650.019.2670963
ATCTGTG7950.018.8850617
AGGACTA7050.018.852715
CCGATCT291000.018.755844
TTCCGAT296650.018.3385142
TCCGATC299300.018.2325463
CGATCTG101400.018.0402725
Produced by FastQC (version 0.11.5)