FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969400
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111521.1504023107076542No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39280.40519909222199296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38850.40076335877862596No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15820.16319372807922428No Hit
GATCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14030.14472869816381265No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13130.13544460491025376No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12970.1337940994429544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGGT6600.028.5877917
GATCTGG27500.026.5548826
CGATCTG77250.026.4146675
TAACGTG808.768194E-526.2054775
CGATCTT33050.025.6900865
AGGTGTA8550.025.42353870
TAGGACA700.001238434524.9575964
GATCTTG11000.024.4584456
TGTACTT1001.4166035E-524.4584455
ATCTAGC4950.023.9996267
CCGATCT206550.023.5305864
GATCTTT11300.023.4998956
ATCTGGG11600.023.4945667
TTCCGAT210700.023.2978442
ATCTAGG5250.023.2937567
AATAGTG750.001850748623.2937565
TCCGATC212250.023.0947843
ATCTTGC5100.022.6086467
ATCTGGC8650.022.2165897
CTTCCGA226900.021.9732061
Produced by FastQC (version 0.11.5)