FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581194
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46150.7940549971266049No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20210.34773242669401266No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19010.3270852761728442No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14840.2553364281117837No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11460.1971802874771591No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9120.15691834396088053No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC6800.1170005196199548No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC6730.11579610250621997No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC6550.11269902992804468No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6450.11097843405128065No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5900.10151515672907842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA451.7008606E-646.5982741
TTCTACT450.004337046831.0655172
CCAATAA500.007221679727.98779327
GGTCCAA500.00725445627.96184511
TATAGGA658.0211E-426.8836212
GTCCTAG700.001236056224.9633621
AGACGTA851.3122906E-424.69256424
ATCTGGG5500.022.2423767
TATATCT800.002687733621.8429434
GTTCCCA800.002687733621.8429431
TCTATAG800.002687733621.8429433
CTTTTTT4050.020.7124799
TATGCTC850.003792697320.58350429
TGGGTAT850.003817693620.5601810
TCTACTC850.003819972520.5580643
TAGCTTG850.003819972520.5580645
TACACGG850.003819972520.5580645
AGGTGTA4350.020.14355570
TAGGTGT6100.020.11053570
AGAATTT1055.499252E-419.9789217
Produced by FastQC (version 0.11.5)