FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529460
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35710.6744607713519435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28790.5437615683904355No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13670.2581875873531523No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8790.16601820723000793No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5740.10841234465304272No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5380.10161296415215502No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC305.91016E-446.610923
ATCTTAC1201.8189894E-1234.962157
GATCTTA1650.033.9026956
ACTATCT652.116703E-532.272765
GGGTACC450.00431408431.09861416
TTTCGGA2850.030.6650831
CGATCTG21550.029.5272035
TATTAGA853.9886727E-628.78912
CTATCTT500.007243787427.969726
TAGCGTC500.007243787427.969725
CTAGACT500.00724779627.9665554
TTCCGAT56800.026.9571972
CCGATCT55650.026.760454
ATAGTGT808.709302E-526.2275588
GTACTAT1207.4742275E-826.2186431
TCCGATC58450.026.1962153
GATCTGG9100.025.3571646
CTTCCGA61700.025.0429841
TAGACGG700.001233109424.9729675
CTGGGGT1551.3023964E-924.809044
Produced by FastQC (version 0.11.5)