FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571872
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54050.9451415701415701No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44360.775698058306754No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16990.2970944547031504No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9360.1636729897599463No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6500.11366179844440713No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATAC350.001262989639.9486057
ATTCCTA552.9821176E-431.83402845
TGAGTGG553.009786E-431.7839610
TGCTACG553.009786E-431.7839610
CTGAGTG553.0116382E-431.7806289
TTCTAAT450.00432869231.07764816
GATCTTT4100.030.6922196
CGATCTG27300.028.8090885
GAGTCTT755.5306184E-528.03750267
GATACTC500.00721084127.99630532
CTTTTTT3500.027.9669539
TAGACTT500.00725157727.9640226
TCTTTTT3550.027.5730538
CGATCTT11450.027.4755675
CCGATCT64750.027.2082374
TCTAGTG658.0075214E-426.89138
TCCGATC66800.026.6872223
ATCTTTT3800.026.6762077
TTCCGAT66750.026.6548442
TAGTGTA5400.026.61526170
Produced by FastQC (version 0.11.5)