FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897255
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41690.46463937230776087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23910.2664794289248876No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT15650.17442087255016692No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14680.16361012198315975No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA14570.1623841605786538No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA14220.15848337429158935No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA12360.13775348145176122No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG11730.13073206613504523No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC11470.12783433917894022No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT11010.1227075914873698No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA10640.11858390312675883No Hit
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG10640.11858390312675883No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA10120.11278844921454882No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10010.11156248781004285No Hit
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC9980.11122813469972305No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC9620.10721589737588534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA2850.030.75975870
CTAGGGG1052.0600055E-829.94653
GTACGAA500.00727424727.9482041
ACGGAGT658.040137E-426.8750657
GGTCCAA3650.025.84768711
ACTAACG1101.012424E-625.4651638
GTAGGGC700.001238981624.9554188
TATAAAG700.001238981624.9554182
ACGTCAA1601.8462742E-924.07896845
ACGTTAC1352.3074972E-723.33838733
ATCATAG3450.023.31814224
TGTTACT750.001841536323.31347722
GGAGTTA750.001851564923.2917239
TTACACG750.001851564923.2917234
AGGTAGC6900.022.7853817
AATCAGG5700.022.72725536
TCGGGTA5150.022.4140825
TCTAGGG1253.1378931E-622.3600542
GTCTTAC5000.022.3585621
AAGGTAG7050.022.3005856
Produced by FastQC (version 0.11.5)