FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575690
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49170.8541055081728014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33260.5777414928173148No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19960.3467143775295732No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17510.3041567510291997No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13360.23206934287550593No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10640.18482169223019335No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC9490.1648456634647119No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC8510.14782261286456252No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8350.1450433392971912No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7530.13079956226441314No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC7440.12923622088276676No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6970.12107210477861349No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6700.11638208063367438No Hit
CGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6240.10839166912748181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGA200.006915893452.56453762
AACGTAC200.006953100752.4932724
ATTAGAA459.297337E-538.815093
TACAATA450.00434398331.0553078
TTTCGGA1151.4861143E-930.3770281
TATTAGA703.532155E-529.9430712
ATAGCAC500.00716680228.03149261
CAAGAGT500.00726229927.95559714
ACGTAGA500.00726229927.95559711
TAACCCC657.895654E-426.95617165
ACATAGC658.0363924E-426.8747838
GAATTGG658.0412824E-426.8719861
CATGTTA1207.437666E-826.2329621
TACTATA808.7729575E-526.2001882
GCTAACC700.001216764425.03073163
CAGATAC851.3244375E-424.6593
ACCGTTA1502.5183908E-823.2914818
ATCTGTG2850.023.2914817
CATAGGT1202.200606E-623.2890554
ATCTGGG4900.022.8161457
Produced by FastQC (version 0.11.5)