FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635479
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34200.5381767139433404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22910.3605154536971324No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12090.19025018922733875No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6870.10810742762546047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT40150.035.4750444
TTCCGAT40450.035.470852
TCCGATC41600.034.4902843
ATCTGAG2700.032.3300447
GTAGAAC553.0368878E-431.7362523
CGATCTG22000.031.5835135
CTTCCGA45600.031.5413821
GATCTGG9500.028.6682386
GTATTAA1103.2592652E-828.5626281
TCTAACG500.007284811727.9384168
ATAATAC500.007298209227.9279023
ATCTAAC658.0654974E-426.8588037
TAGGCCC658.074369E-426.853754
ATCTGGG4650.026.2811977
CTAGAAG808.815284E-526.182413
CGATCTA8250.025.8170095
ATATAAG1659.822543E-1125.3890042
CGATCTC10400.024.8443935
TATAAGA1601.9153958E-924.0005423
GATCTAG3300.023.2776326
Produced by FastQC (version 0.11.5)