FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335748
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28980.8631473605203903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20400.6075985560599021No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12070.35949581233544203No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8670.2582293863254584No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7380.2198077129275528No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6850.20402206416717297No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5450.16232412404541502No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4010.11943481420589253No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3840.11437149290539333No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3690.1099038564637764No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3670.10930817160489413No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3630.10811680188712965No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3470.10335132301607158No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3390.10096858358054255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAGTT350.00126253139.945845
CGGAGTA400.002426069234.97130224
GTTATGG400.002432441634.952619
TATAGGA400.002434568734.9463842
GTATAAT400.002434568734.9463841
ACAGTAC450.00433159131.0689878
ATACAGT450.00433159131.0689876
AGCTAGG604.951972E-429.18957748
CTAGGAG604.951972E-429.18957750
TTTCGGA605.0196605E-429.1219841
AGTTATG500.00724901927.9620888
ACTATAA500.007255327427.9571062
GCTAGGA657.9078716E-426.94422749
ATCTTTG658.007398E-426.8866257
CGGTGTA3000.026.86400870
TAGGGGC700.00123395324.9661484
TATACAG700.00123395324.9661485
ATAGGGG1551.3023964E-924.8006613
TTAGGAC1001.4105248E-524.4624673
ATCTATG1052.0549387E-523.301747
Produced by FastQC (version 0.11.5)