FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833514
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54730.6566176452945002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22930.2751003582423331No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20120.2413876671537611No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC9170.11001614849900541No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC9120.10941627855081018No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT8870.10641692880983403No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA8770.10521718891344357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG32900.033.987685
TTCCGAT73000.033.5027352
CCGATCT72200.033.1480874
TCCGATC74050.032.9333153
ATCTGTG2850.031.8783577
CTTCCGA80150.030.6012151
GATCTGG15150.030.2152446
ATCTGGG8300.028.6284777
TCTTTTT6300.027.1783438
GTGTAAT658.038235E-426.8758221
CTTTTTT6300.026.0690219
TCTGTCC1900.025.7479048
CGATCTT17750.025.395625
CGATCTA15000.025.159385
ATCTTTT7050.024.7826827
GGTCCAA3700.024.55671311
GATCTAG4850.024.4965326
GATCTCA5300.024.3945776
TCTGACC1752.055458E-1023.9613138
ATCTGAG3800.023.9087687
Produced by FastQC (version 0.11.5)