FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327162
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26360.8057170453781306No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11770.3597606078945599No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8960.27387043727572274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8850.27050818860381093No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7150.21854616367426535No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC6240.19073119738844976No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6080.185840653865669No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5720.17483693093941227No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5670.1733086360885433No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC5610.1714746822675005No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5390.16475018492367693No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4280.1308220392343854No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3820.11676172660639071No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3450.1054523447099602No Hit
CGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3360.10270141397839602No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3340.10209009603804843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTT150.002221106670.0157331
GAACTAC252.3765444E-456.05358555
AACGTAT350.001261747239.9505419
AACATTT450.004298047631.11810335
TGAGCGG450.004336660731.06129610
TTCTATA450.004340537431.0556262
TAACTAC450.004340537431.0556264
GGACCGC802.494031E-630.5703836
GTCCTAA1052.050001E-829.9464971
GGCCACA604.930813E-429.21061562
TGTTACG605.021386E-429.11996516
TTGTTAC605.021386E-429.11996515
TTTCGGA605.026756E-429.1146491
AGTTAGA500.00716095828.03192361
TAGGAGT500.007193019628.0062929
GAACGTA500.007251017327.9602718
TACCATG1253.596142E-927.95516610
CATGAGT500.007257484427.95516614
ATGGAGT500.007257484427.95516615
CATAGGT500.007257484427.95516616