FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662058
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55870.8438837684915823No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48750.7363403206365605No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20380.30782801506816626No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13460.20330545057985855No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13230.19983143470813733No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11050.16690380601095373No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8720.13171051478873452No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC8610.13004902893704176No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8310.12551770388697062No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC7810.11796549547018538No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7580.11449147959846419No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7200.1087518012017074No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAG652.124402E-532.2577741
GTATACG652.124402E-532.2577741
TAAGGAT450.004337334531.0658514
TAAGACA450.004337334531.0658514
CGTAAAT1151.4679244E-930.4125123
CTCCTAG604.981203E-429.16644130
ACTGTCC605.0209556E-429.126878
AAATTAC1253.5634002E-927.99217826
TAGGCAT500.007258584727.9592655
TCTTAGG500.0072617927.9567382
TATACGG500.0072617927.9567382
CGCGTAA1401.1998054E-824.97718221
GTATGTA851.3175674E-424.67886716
GCGCGTA1601.880835E-924.03836420
ATGTATG901.9426395E-423.3057114
TAGACAG1052.0612262E-523.2993875
ATAACTA750.001847412823.2993875
TTCGGAA1652.7030183E-923.2972812
CTCGGAC1253.0632127E-622.42013453
TATAGTA2207.2759576E-1222.2383142
Produced by FastQC (version 0.11.5)