FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524176
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32700.6238362687341656No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14600.2785324013308507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12510.23866029730472207No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10240.19535423216629527No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10140.19344647599279632No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8020.15300204511461798No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7560.1442263667165227No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5830.11122218491499039No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5720.1091236531241415No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5500.10492658954244377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA450.0043501631.0456811
GTATATC450.0043501631.0456811
TTAGACT605.041053E-429.1053284
AGAAACG2100.028.3872267
GGTCCAA1601.8189894E-1228.38093611
CCTAGGT500.00714301728.05003465
ACCCCTA500.00715502128.04038853
TCTAGCG755.6564953E-527.9443078
TCTAACG500.007280001427.9411143
CTAGGAC2950.027.230753
GAGAAAC2200.027.09689166
GGAATCA2850.027.0162634
TCTAGGA3400.026.7084182
TAGGACT2900.026.4958844
TTCCGGA2950.026.1363549
GGGTAAC2850.025.76760925
AGGACTA3000.025.612695
GGTAACG2900.025.3233426
TAACGGG2950.024.89982628
AACGGGT3100.024.8091422