FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779275
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50190.6440601841455199No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20970.2690962753841712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17600.22585095120464535No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT14250.1828622758333066No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG11730.15052452600173238No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA11550.1482146867280485No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT10710.13743543678419043No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10480.13448397549003882No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA9810.12588624041577107No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT9630.12357640114208719No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC9480.12165153508068398No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9350.11998331782746784No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC8990.11536363928010009No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG8870.11382374643097752No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT7860.10086298161752913No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA605.042232E-429.1067854
CCTTACA1352.6557245E-1028.4599692
TTATAGG959.540583E-625.7365283
TTACTCA700.001239087924.95443311
TCTAGGG1401.2152668E-824.9486732
GTATAAA851.326543E-424.6551591
GGTCCAA3150.024.39988911
GTATTAA901.9546482E-423.2854291
TATAGGT750.001854212323.2854294
GATCTGG13800.023.0340986
GTCCTAG5350.022.8501871
CTTACAC2450.022.8102153
TTTCGGA2300.022.7792241
CTTGGGG2300.022.7792243
AGTGTAG3400.022.69660269
GGTACAA5100.022.6808357
GTGTAGA3450.022.36766470
TCTAGGC952.8155776E-422.0632748
ATACAGC952.8170436E-422.0615796
GTATATA800.002697905521.8300911
Produced by FastQC (version 0.11.5)