FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542775
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165401.0720941161219233No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99400.6442935619257507No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78080.5061010192672295No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC70170.45482977102947614No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69180.4484127627165335No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC62700.4064105264863639No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC58930.3819740402845522No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57350.371732754290159No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC53560.3471666315567727No Hit
GAGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40710.26387516002009365No Hit
GACGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40350.2615417024517509No Hit
GCACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38750.25117077992578307No Hit
CGTGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34160.22141919592941292No Hit
CACGTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32080.20793699664565474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31080.20145517006692487No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA27990.18142632593864952Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25510.1653513960233994No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC23980.15543420135794267Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23830.15446192737113318No Hit
TCCCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21960.1423409116689083No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18400.1192656090486299No Hit
TCCCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17960.11641360535398874No Hit
TCTCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17070.11064477969891917No Hit
GGGAGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16810.1089595047884494No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC16450.10662604722010663No Hit
TCCGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16140.10461668098070036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCA1251.03682396E-1030.86161869
GTATTAA605.0416077E-429.1100711
TACCTAT1352.6375346E-1028.46983310
ACATAAT2450.027.19715969
AGTATCG808.568012E-526.29634967
TTTCGGA2550.026.0278281
GATACTC77700.023.42514870
CGAGTAA1652.6011548E-923.38001470
ATGTTAT3450.023.31165922
TAGCCCA901.9448198E-423.305319
GGGTACA750.001847685123.30166816
TCGATTC4300.022.81043245
GAAACTA1102.879016E-522.31728670
GGACTGC1102.9644169E-522.2329716
TGCACAA3150.022.19206416
ATAGCAA1905.493348E-1022.14948770
TCCATAA800.002697558421.8325542
ATACCTA1454.6859168E-721.687059
TTGTGTA11650.021.37314670
TGTAAGG1154.182146E-521.2630082
Produced by FastQC (version 0.11.5)