FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210194
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18840.8963148329638335No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5910.28116882499024715No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4600.21884544753894022No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4310.20504866932452878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3840.18268837359772402No Hit
GATCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2330.11084997668820233No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2160.10276221014871975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAAGC150.0022355669.8908166
CCGCATT252.3870745E-455.99225229
ATCCGAC200.00693044452.5226543
CTGGCCG352.0994958E-549.922019
ATCTGGC703.8744474E-1044.929817
ATCTGGG1750.041.934497
GATCTTT602.6149246E-740.769646
GATCTGG3450.039.50356
CGGGAAC901.05501385E-1038.85032315
TTAGGTG556.6471293E-638.1982945
TCTGTGC556.7387173E-638.1222658
CGATCTG10350.037.1400455
CGATCTA5650.037.110175
CGCGGTG400.00240876935.015152
TTCCGAT24850.035.0057682
TCTGCAG400.002425656234.96529410
CGGGTGT400.002425656234.96529415
TCTGGCC501.7203226E-434.9454048
TCCGATC25350.034.315323
CCGATCT25350.034.315324