FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671505
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75411.1229998287428984No Hit
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49390.7355120215039351No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16730.24914185300183916No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13310.19821148018257495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTA350.001266332639.9283335
CTAGGAC556.7800793E-638.1100043
GATCTTT5100.037.6774756
CGATCTG26200.035.870735
TTCCGAT60300.034.9341742
TATATGC400.002440810734.934174
CCGATCT59400.034.8753554
TCCGATC61050.034.3905563
CGATCTT12600.034.3827325
CTAGGCG751.5110381E-632.608149
GATCTAG4550.032.249816
ATTATGG553.024723E-431.7583373
CTCTATA450.00434644531.0525951
CTTCCGA68150.030.9102061
TCTGTCC802.5003337E-630.570138
TAGGACT802.5020781E-630.5673984
TCTTTTT4750.030.15648
TGTATAC703.53278E-529.9435733
ATCTTTT5450.029.488367
ATCTAGT1202.3410394E-929.1144087
Produced by FastQC (version 0.11.5)