FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349294
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25660.7346247001093635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16570.47438547470039566No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9950.28486031824193947No Hit
CGTCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7040.20154941109781446No Hit
GAAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6640.19009774001271135No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5930.16977102383665335No Hit
GATCGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5250.15030318299197812No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4610.13198050925581317No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4420.12654096549038918No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3740.10707312464571393No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3710.10621424931433118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAGT200.00692389652.54395348
TAAGAGG501.7075762E-434.9992336
GAACGTA400.002428980834.96320718
TAATCAC400.002439219334.9332476
CCGTACG450.004292855531.1264643
TCACATA450.00433634831.0624229
TTTAATC450.004343630331.0517754
TTCGGAA703.5276833E-529.9427832
TAACTTA1301.6552804E-1029.62999344
GAGACAA604.921124E-429.22121269
GGTAGAC604.931048E-429.21116464
ATTTAAT605.0311984E-429.111043
GTATGCT500.00716027128.03307552
ATCCTAA500.00725695727.9561829
AGGTACG500.007263033727.9513918
CATCCTA500.007263033727.9513918
TATAAGA755.6469675E-527.9465983
TAGAGTT1154.8872607E-827.38599830
CCTGTTT658.0260827E-426.8763358
ATAGGCC658.034125E-426.8717293
Produced by FastQC (version 0.11.5)