FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452734
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41060.9069343146306662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30340.6701506845079008No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13410.2962004179054368No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9470.2091735986252413No Hit
GGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5430.11993797682524397No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA5160.11397421002177878No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4740.10469723943861076No Hit
GATCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4610.10182579616286826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAGT556.768061E-638.115128
TTCCGAT47850.036.6499372
CGATCTG21700.036.222155
CCGATCT48350.035.981924
TCCGATC48950.035.7549743
CCACTAG400.002403696335.04054657
AAACACT400.00243663834.94346616
TCTTGTC400.002438215534.938868
GTGTTAA501.7273107E-434.9342541
TAGACTG400.00243979434.9342545
TCTAGAC806.336268E-834.9342543
CGATCTA9000.034.1579365
CTTCCGA53500.032.844731
TAATATT652.1275036E-532.2470054
CGATCTT8700.032.1234475
GATCTGG10700.031.342886
GATCTTG3200.030.567476
ATCTGGT1850.030.2134087
CGATCTC12900.029.7888975
GGGTCAC605.0253916E-429.11955816