FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adR3wtZT14s48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adR3wtZT14s48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500729
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29010.5793552999726399No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21490.4291742639232No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10320.2060995069189122No Hit
GTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10000.19970882453382968No Hit
CTTCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6970.1391970507000793No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6620.13220724184139523No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6450.12881219182432013No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC6110.12202209179016993No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6110.12202209179016993No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5480.10944043584453866No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5440.10864160054640334No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA5420.10824218289733568No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5350.10684422112559888No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT5260.1050468417047944No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5050.100852956389584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT33100.034.821362
TCCGATC33850.034.0498433
CCGATCT33700.033.8904724
CTTCCGA36550.031.5345291
CGATCTG17750.030.1059885
GATCTGG7500.029.3385816
TCTAGGG605.0402165E-429.1057342
GTTTAGA755.6596975E-527.9415051
GTCATAG500.007279096627.9415041
GTACATG500.007279096627.9415041
ATTGGGC500.007279096627.9415043
ACAGGGA906.264154E-627.16535213
GATCTAG3000.026.7772756
TCTAGGC808.781643E-526.195168
TCTGGTC1207.5373464E-826.195168
ATCTAGC959.532494E-625.7355987
ATCTGGG3400.025.681537
CGATCTC10400.025.523495
TCAAGTC851.2978744E-424.73153548
ATCTCGT1601.900844E-924.012237
Produced by FastQC (version 0.11.5)